Product Information

Contents: Affinity Purified anti-human CD154 (CD40 Ligand, CD40L)
Catalog Number: 14-1548
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 24-31
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Human PBMC samples were activated with TPA and Ionomycin for 6 hours and stained with 0.5 µg of purified anti-CD154 followed by anti-mouse IgG-FITC. Viable cells were analyzed and gated for small cells. Markers were set based on the autofluorescence sample.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1548	FITC anti-human CD154 (CD40 Ligand, CD40L)	488	518	FC
12-1548	PE anti-human CD154 (CD40 Ligand, CD40L)	488	575	FC
17-1548	Allophycocyanin (APC) anti-human CD154 (CD40 Ligand, CD40L)	633	660	FC
27-1548	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD154 (CD40 Ligand, CD40L)	633	779	FC
14-1548	Affinity Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
16-1548	Functional Grade* Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the Cynomogus monkey CD154.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro. (Please use Functional Grade purified 24-31, cat. 16-1548, in functional assays.)

Applications Tested

The 24-31 antibody has been tested by flow cytometric analysis of resting and activated human PBMC. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brams, P., A. Black, et al. 2001. A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation. Int Immunopharmacol 1(2): 277-94.

Related Products

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