Product Information

Contents: Affinity Purified anti-mouse CD152 (CTLA-4)
Catalog Number: 14-1522
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: UC10-4B9
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1522	Affinity Purified anti-mouse CD152 (CTLA-4, CTLA4)	N/A	N/A	FC IP
13-1522	Biotin anti-mouse CD152 (CTLA-4, CTLA4)	N/A	N/A	FC
12-1522	PE anti-mouse CD152 (CTLA-4, CTLA4)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UC10-4B9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in in vitro functional studies. (Please use Functional Grade purified UC10-4B9, cat. 16-1522, in functional assays.)

Applications Tested

The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

References

Walunas, T. L., D. J. Lenschow, et al. 1994. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1(5): 405-13.
June, C.H., J.A. Bluestone, L.M. Nadler and C.B. Thompson (1994) The B7 and CD28 receptor families. Immunol Today 15: 231-331.
Krummel, M. F. and J. P. Allison (1995). "CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation." J Exp Med 182(2): 459-65.

Related Products

Cat. 12-1521  PE anti-mouse CD152 (CTLA-4, CTLA4) (clone 9H10)
Cat. 14-1521  Affinity Purified anti-mouse CD152 (CTLA-4, CTLA4) (clone 9H10)
Cat. 16-1521  Functional Grade Purified anti-mouse CD152 (CTLA-4, CTLA4) (clone 9H10)
Cat. 11-4111  FITC Anti-Armenian Hamster IgG
Cat. 13-4113  Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4444  Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 14-4888  Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)