Product Information

Contents: Affinity Purified anti-rat CD152 (CTLA-4)
Catalog Number: 14-1520
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: WKH203
Isotype: Mouse IgG1

Staining of unstimulated (left) and 3-day ConA activated (right) Lou Rat splenocytes with FITC anti-hu/mo/rat ICOS (C398.4A) (cat. 11-9949) and 0.5 μg Purified anti-mouse CTLA-4 (WKH203) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and Streptavidin-FITC (cat. 11-4311). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1520	Phycoerythrin (PE) anti-rat CD152 (CTLA-4, CTLA4)	488	575	FC
13-1520	Biotin anti-rat CD152 (CTLA-4, CTLA4)	N/A	N/A	FC
14-1520	Affinity Purified anti-rat CD152 (CTLA-4, CTLA4)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The WKH203 monoclonal antibody reacts with rat CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The WKH203 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This WKH203 antibody has been tested by flow cytometric analysis of unstimulated and ConA-activated (3 days) rat splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

References

Lin CH, Hunig T. (2003) Efficient expansion of regulatory T cells in vitro and in vivo with a CD28 superagonist. Eur J Immunol. 33(3):626-38.

Elflein K, Rodriguez-Palmero M, Kerkau T, Hünig T. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy. Blood, 102( 5):1764-1770.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control