Product Information

Contents: Affinity Purified anti-human CD150 (SLAM, IPO-3)
Catalog Number: 14-1509
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: A12 (7D4)
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Staining of normal human peripheral blood cells with 1 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 1 μg of Purified anti-human CD150 (A12/7D4) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1509	Affinity Purified anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FA FC IHC
16-1509	Functional Grade* Purified anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FA FC
13-1509	Biotin anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FC
11-1509	FITC anti-human CD150 (SLAM, IPO-3, IPO3)	488	518	FC
12-1509	PE anti-human CD150 (SLAM, IPO-3, IPO3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. A12 (7D4) has been reported for use in flow cytometric analysis, and immunohistochemical staining. A12 has also been reported in vitro functional studies. (Please use Functional Grade purified A12 (7D4), cat. 16-1509, in functional assays.)

Applications Tested

The A12 (7D4) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cocks, B. G., C. C. Chang, et al. (1995). "A novel receptor involved in T-cell activation." Nature 376(6537): 260-3.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control