Product Information

Contents: Affinity Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen)
Catalog Number: 14-1508
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio13G3-19D (13G3-19D)
Isotype: Mouse IgG2a, κ

Staining of BALB/c splenocytes with 0.25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.25 μg of Purified anti-mouse Ly108 (13G3-19D) (colored histogram) followed by Biotin Anti-Mouse IgG2a and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1508	Affinity Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	N/A	N/A	FC IP WB
16-1508	Functional Grade* Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	N/A	N/A	FA FC IP WB
13-1508	Biotin anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	N/A	N/A	FC
12-1508	Phycoerythrin (PE) anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.

Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

Applications Tested

This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. Immunogenetics. 2000 Nov;52(1-2):63-72.

Fraser CC, Howie D, Morra M, Qiu Y, Murphy C, Shen Q, Gutierrez-Ramos JC, Coyle A, Kingsbury GA, Terhorst C. Identification and characterization of SF2000 and SF2001, two new members of the immune receptor SLAM/CD2 family. Immunogenetics. 2002 Feb;53(10-11):843-50.

Howie D, Laroux FS, Morra M, Satoskar AR, Rosas LE, Faubion WA, Julien A, Rietdijk S, Coyle AJ, Fraser C, Terhorst C. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005 May 15;174(10):5931-5.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724  Affinity Purified Mouse IgG2a, K Isotype Control