mouse CD140a (PDGFRa) antibody: purified antibody

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Contents: Affinity Purified anti-mouse CD140a (PDGFRα)
Catalog Number: 14-1401
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: APA5
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-1401-81	50 ug	$60
14-1401-82	100 ug	$95

*International customers: Please contact your distributor for region specific pricing.

Staining of NIH/3T3 cells with 0.5 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.5 μg of Purified anti-mouse CD140a (APA5) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1401	Affinity Purified anti-mouse CD140a (PDGFRa)	N/A	N/A	FC IH/F WB
16-1401	Functional Grade* Purified anti-mouse CD140a (PDGFRa)	N/A	N/A	FA FC IH/F WB
13-1401	Biotin anti-mouse CD140a (PDGFRa)	N/A	N/A	FC
12-1401	Phycoerythrin (PE) anti-mouse CD140a (PDGFRa)	488	575	FC
17-1401	Allophycocyanin (APC) anti-mouse CD140a (PDGFRa)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the α chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of α/α, β/β, or heterodimers of α/β and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified APA5 in functional assays.)

Applications Tested

The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Takakura, N., H. Yoshida, et al. (1996). "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation [comment]." J Invest Dermatol 107(5): 770-7.
Takakura, N., H. Yoshida, et al. (1997). "PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5." J Histochem Cytochem 45(6): 883-93.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321    Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)