Product Information

Contents: Affinity Purified anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)
Catalog Number: 14-1331
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 13A4
Isotype: Rat IgG1, κ

Staining of BALB/c bone marrow cells with 0.5 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (open histogram) or 0.5 μg of Purified 13A4 (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813) and SAv-PE (cat. 12-4317). Cells in the myeloid gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1331	Affinity Purified anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	N/A	N/A	FC IH/F IHC IP WB
16-1331	Functional Grade* Purified anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	N/A	N/A	FC
13-1331	Biotin anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	N/A	N/A	FC
11-1331	FITC anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	488	518	FC
12-1331	Phycoerythrin (PE) anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	488	575	FC
17-1331	Allophycocyanin (APC) anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	633	660	FC
36-1331	Functional Grade* Biotin anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	N/A	N/A	FC
53-1331	Alexa Fluor® 488 anti-mouse Prominin I (Prominin-I, CD133, AC133, AC-133)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 13A4 monoclonal antibody recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural & endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown. The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 13A4 antibody has been reported for use in flow cytometric analysis (~0.25-1 μg/million cells), immunoprecipitation (~10-25 μg/ml), immunoblotting (WB) (~1-5 μg/ml), and immunohistochemical staining (~1-10 μg/ml).

Applications Tested

The 13A4 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Pfenninger CV, Roschupkina T, Hertwig F, Kottwitz D, Englund E, Bengzon J, Jacobsen SE, Nuber UA. CD133 is not present on neurogenic astrocytes in the adult subventricular zone, but on embryonic neural stem cells, ependymal cells, and glioblastoma cells. Cancer Res. 2007 Jun 15;67(12):5727-36. (13A4, Immunofluorescence, PubMed)

Sugiyama T, Rodriguez RT, McLean GW, Kim SK. Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):175-80. (13A4, FC, PubMed)

Lamerato-Kozicki AR, Helm KM, Jubala CM, Cutter GC, Modiano JF. Canine hemangiosarcoma originates from hematopoietic precursors with potential for endothelial differentiation. Exp Hematol. 2006 Jul;34(7):870-8. (13A4, canine cross-reactivity, PubMed)

Kania G, Corbeil D, Fuchs J, Tarasov KV, Blyszczuk P, Huttner WB, Boheler KR, Wobus AM. Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors. Stem Cells 2005 Jun-Jul;23(6):791-804. (13A4, IHC and WB)

Fargeas CA, Joester A, Missol-Kolka E, Hellwig A, Huttner WB, Corbeil D. Identification of novel Prominin-1/CD133 splice variants with alternative C-termini and their expression in epididymis and testis. J Cell Sci 2004 Aug 15;117(Pt 18):4301-11. (13A4, IHC frozen)

Fargeas CA, Corbeil D, Huttner WB. AC133 Antigen, CD133, Prominin-1, Prominin-2, Etc.: Prominin Family Gene Products in Need of a Rational Nomenclature.Stem Cells. 2003;21(4):506-8.

Sawamoto K, Nakao N, Kakishita K, Ogawa Y, Toyama Y, Yamamoto A, Yamaguchi M, Mori K, Goldman SA, Itakura T, Okano H. Generation of Dopaminergic Neurons in the Adult Brain from Mesencephalic Precursor Cells Labeled with a nestin-GFP Transgene. J Neurosci. 2001 Jun 1;21(11):3895-903.

Röper K, Corbeil D, Huttner WB. Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane.Nat Cell Biol. 2000 Sep;2(9):582-92.

Corbeil D, Röper K, Hellwig A, Tavian M, Miraglia S, Watt SM, Simmons PJ, Peault B, Buck DW, Huttner WB. The Human AC133 Hematopoietic Stem Cell Antigen Is also Expressed in Epithelial Cells and Targeted to Plasma Membrane Protrusions. J Biol Chem. 2000 Feb 25;275(8):5512-20.

Corbeil D, Röper K, Hannah MJ, Hellwig A, Huttner WB. Selective localization of the polytopic membrane protein prominin in microvilli of epithelial cells - a combination of apical sorting and retention in plasma membrane protrusions. J Cell Sci. 1999 Apr;112 ( Pt 7):1023-33.

Weigmann A, Corbeil D, Hellwig A, Huttner WB. Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells.Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12425-30.

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