Product Information

Contents: Affinity Purified anti-human CD123 (interleukin-3 receptor α, IL-3Rα)
Catalog Number: 14-1239
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 6H6
Isotype: Mouse IgG1, κ

Staining of TF-1 cells with Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or Purified anti-human CD123 (6H6) (colored histogram) followed by PE Anti-Mouse IgG (cat. 12-4012). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1239	Fluorescein isothiocyanate (FITC) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)	488	518	FC
12-1239	PE anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	575	FC
13-1239	Biotin anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC
14-1239	Affinity Purified anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC IH/F IHC(Paraffin)
17-1239	Allophycocyanin (APC) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	633	660	FC
25-1239	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	760	FC
45-1239	PerCP-Cy5.5 anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 6H6 monoclonal antibody reacts with human CD123, the α chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common β chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 6H6 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

The 6H6 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Herling, M., M. Teitell, R. Shen, L. Medeiros, and D. Jones. 2003. TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood. 101: 5007-5009.

Jaye, D.L., C.M. Geigerman, et al. 2006. Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+CD56+ hematodermic neoplasms. Mod Pathol. 19(12): 1555-62. (IHC paraffin PubMed)

Peduzzi, E., C. Groeper, et al. 2007. Local activation of the innate immune system in Buruli Ulcer lesions. J Invest Dermatol. 127(3) :638-45. (IHC paraffin PubMed)

Assaf C., S. Gellrich, et al. 2007. CD56 lymphoproliferative disorders of the skin: A multicenter study of the cutaneous lymphoma project group of the european organization for research and greatment of cancer (EORTC). J Clin Pathol. 60(9): 981-9. (IHC frozen PubMed)

Xu. W., B. He, et al. 2007. Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLP1. Nature Immunol. 8(3): 294-303. (IHC frozen PubMed)

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Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control