Product Information

Contents: Affinity Purified anti-mouse IL-21R (Interleukin-21 Receptor, IL-21 Receptor)
Catalog Number: 14-1219
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio4A9
Isotype: Rat IgG2a, κ

Staining of 2 day anti-CD3 (cat 16-0031) stimulated BALB/c splenocytes with APC anti-mouse CD4 (GK1.5) (cat. 17-0041) and Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (left) or 0.25 μg of Purified anti-mouse IL-21R (eBio4A9) (right) followed by Biotin Anti-Rat IgG (cat. 13-4813) then SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1219	Affinity Purified anti-mouse IL-21R (Interleukin-21 Receptor, IL-21 Receptor, IL21R)	N/A	N/A	FC
13-1219	Biotin anti-mouse IL-21R (Interleukin-21 Receptor, IL-21 Receptor, IL21R)	N/A	N/A	FC
12-1219	Phycoerythrin (PE) anti-mouse IL-21R (Interleukin-21 Receptor, IL-21 Receptor, IL21R)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The IL-21 receptor (IL-21R) combines with the common γ-chain to form a functional receptor for IL-21. IL-21R is expressed on several lineages including both resting and activated B, T, natural killer and dendritic cells. Binding of its ligand, IL-21, in these cells results in the activation of the Jak/Stat signal transduction pathway. The biological effects of ligand binding are diverse and depend on the cellular context and differentiation state of the cell. In the mouse, IL-21 binding to IL-21R induces the apoptosis of resting and activated B cells, enhances anti-CD3-mediated proliferation of T cells, and inhibits the maturation of dendritic cells and the IL-15-mediated expansion of NK cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4A9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio4A9 antibody has been tested by flow cytometric analysis of CD3 stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Jin H, Carrio R, Yu A, Malek TR. Distinct activation signals determine whether IL-21 induces B cell costimulation, growth arrest, or Bim-dependent apoptosis. J Immunol. 2004 Jul 1;173(1):657-65.

Vosshenrich CA, Ranson T, Samson SI, Corcuff E, Colucci F, Rosmaraki EE, Di Santo JP. Roles for common cytokine receptor gamma-chain-dependent cytokines in the generation, differentiation, and maturation of NK cell precursors and peripheral NK cells in vivo. J Immunol. 2005 Feb 1;174(3):1213-21.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)