human CD117 (c-Kit, cKit) antibody: purified antibody

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Contents: Affinity Purified anti-human CD117 (c-Kit, cKit)
Catalog Number: 14-1179
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: YB5.B8
Isotype: Mouse IgG1, κ
HLDA No.: V C009

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-1179-80	25 ug	$75
14-1179-82	100 ug	$175

*International customers: Please contact your distributor for region specific pricing.

Surface staining of TF-1 cells with anti-human CD117 (YB5.B8) PE. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1179	PE anti-human CD117 (c-Kit)	488	575	FC
13-1179	Biotin anti-human CD117 (c-Kit)	N/A	N/A	FC
14-1179	Affinity Purified anti-human CD117 (c-Kit)	N/A	N/A	FC IH/F IP
17-1179	Allophycocyanin (APC) anti-human CD117 (c-Kit)	633	660	FC
57-1179	Pacific Blue® anti-human CD117 (c-Kit)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. YB5.B8 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Lerner NB, Nocka KH, Cole SR, Qiu FH, Strife A, Ashman LK, Besmer P. Monoclonal antibody YB5.B8 identifies the human c-kit protein product. Blood 1991 May 1;77(9):1876-83
Okayama Y, Hunt TC, Kassel O, Ashman LK, Church MK. Assessment of the anti-c-kit monoclonal antibody YB5.B8 in affinity magnetic enrichment of human lung mast cells.
J Immunol Methods 1994 Mar 10;169(2):153-61
Ashman LK, Buhring HJ, Aylett GW, Broudy VC, Muller C. Epitope mapping and functional studies with three monoclonal antibodies to the c-kit receptor tyrosine kinase, YB5.B8, 17F11, and SR-1. J Cell Physiol 1994 Mar;158(3):545-54

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