human CD116 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human CD116 (GM-CSFRα)
Catalog Number: 14-1169
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 4H1
Isotype: Mouse IgG1, κ
HLDA No.: N/A

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14-1169-80

25 ug

14-1169-82

100 ug

Surface staining of normal human peripheral blood cells with anti-human CD116 (4H1) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1169	Affinity Purified anti-human CD116 (GM-CSFR alpha)	N/A	N/A	FC
13-1169	Biotin anti-human CD116 (GM-CSFR alpha)	N/A	N/A	FC
11-1169	FITC anti-human CD116 (GM-CSFR alpha)	488	518	FC
12-1169	PE anti-human CD116 (GM-CSFR alpha)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 4H1 monoclonal antibody reacts with the human CD116 molecule, the α subunit of GM-CSF receptor. The α subunit associates with the common β chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR α chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 4H1 has been reported for use in flow cytometric analysis.

Applications Tested

The 4H1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sun, Q., K. Jones, et al. (1999). Simultaneous antagonism of interleukin-5, granulocyte-macrophage colony-stimulating factor, and interleukin-3 stimulation of human eosinophils by targetting the common cytokine binding site of their receptors. Blood 94(6): 1943-51.
Woodcock, J. M., B. J. McClure, et al. (1997). The human granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor exists as a preformed receptor complex that can be activated by GM-CSF, interleukin-3, or interleukin-5. Blood 90(8): 3005-17.
Lopez, A. F., M. A. Vadas, et al. (1991). Interleukin-5, interleukin-3, and granulocyte-macrophage colony-stimulating factor cross-compete for binding to cell surface receptors on human eosinophils. J Biol Chem 266(36): 24741-7.

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