mouse CD115 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse CD115 (FMS, CSF-1R)
Catalog Number: 14-1152
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: AFS98
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-1152-81	50 ug
14-1152-82	100 ug
14-1152-85	500 ug

Staining of thioglycolate-induced PEC cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD115 (AFS98) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1152	Affinity Purified anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	N/A	N/A	FA FC IH/F WB
16-1152	Functional Grade* Purified anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	N/A	N/A	FA FC
13-1152	Biotin anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	N/A	N/A	FC
12-1152	PE anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	488	575	FC
17-1152	Allophycocyanin (APC) anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AFS98 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified AFS98, cat. 16-1152, in functional assays.)

Applications Tested

The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Murayama T, Yokode M, et al. 1999. Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation. 99(13): 1740-6.

Sudo T, Nishikawa S, et al. 1995. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 11(12): 2469-76.

Related Products

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Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)