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Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD107a (LAMP-1) Catalog Number: 14-1079 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: eBioH4A3 Isotype: Mouse IgG1, κ Prices for This Product* Cat. No. Size Price Qty Action 14-1079-80 25 ug $55 14-1079-82 100 ug $100 *International customers: Please contact your distributor for region specific pricing.

PBMCs were lysed and 10 µg total protein/lane was immunoblotted using anti-human LAMP1 (eBioH4A3) antibody at 1 µg/ml and revealed with anti-mouse HRP.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1079	Fluorescein isothiocyanate (FITC) anti-human CD107a (LAMP-1)	488	518	IC Flow
12-1079	Phycoerythrin (PE) anti-human CD107a (LAMP-1)	488	575	IC Flow
15-1079	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD107a (LAMP-1)	488	670	IC Flow
51-1079	Alexa Fluor® 647 anti-human CD107a (LAMP-1)	633	668	IC Flow
53-1079	Alexa Fluor® 488 anti-human CD107a (LAMP-1)	488	519	IC Flow
14-1079	Affinity Purified anti-human CD107a (LAMP-1)	N/A	N/A	IC Flow  IHC  WB
13-1079	Biotin anti-human CD107a (LAMP-1)	N/A	N/A	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as LAMP-1 (lysosomal-associated membrane protein 1). CD107a, an approximately 110kDa protein, is highly glycosylated, and along with LAMP-2 (CD107b) is estimated to contribute to approximately 50% of all proteins of the lysosomal membrane. CD107a is expressed intracellularly on many cell types, and on the surface of activated platelets and T cells, as well as some cancer cells. It is reported that surface CD107a binds to CD62E (E-Selectin).

It is recommended to use the brightest fluorochromes (cat. 12-1079) for staining for rather than purified and biotinylated formats. Please see other formats for data comparison.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. Purified anti-human CD107a (LAMP-1) has been reported for use in flow cytometric analysis, immunohistochemistry, and immunoblotting.

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References

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Sarafian V, Jans R, Poumay Y. Expression of lysosome-associated membrane protein 1 (LAMP-1) and galectins in human keratinocytes is regulated by differentiation. Arch. Dermatol. Res. 2006 Jul; 298(2):73-81. (H4A3, IHC, WB, PubMed)

Huynh C, Andrews NW. The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis. EMBO Rep. 2005 Sep;6(9):843-7. (H4A3, FC, IHC, PubMed)

Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brule F, Castronovo V, Wattiaux R, Coninck SW. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells. Int. J. Cancer. 1998 Jan; 75(1):105-111. (H4A3, FC, IHC, PubMed)

Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112.

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Related Products

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Cat. 14-1071    Affinity Purified anti-mouse CD107a (LAMP-1) (clone eBio1D4B (1D4B))
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control