Product Information

Contents: Affinity Purified anti-human CD107b (LAMP-2)
Catalog Number: 14-1078
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioH4B4 (H4B4)
Isotype: Mouse IgG1, κ

PBMCs (P) and Jurkat cells (J) were lysed and 10 µg total protein/lane was immunoblotted using anti-human LAMP2 (eBioH4B4) antibody at 1 µg/ml and revealed with anti-mouse HRP.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1078	Affinity Purified anti-human CD107b (LAMP-2)	N/A	N/A	IC Flow
13-1078	Biotin anti-human CD107b (LAMP-2)	N/A	N/A	IC Flow
11-1078	Fluorescein isothiocyanate (FITC) anti-human CD107b (LAMP-2)	488	518	IC Flow
51-1078	Alexa Fluor® 647 anti-human CD107b (LAMP-2)	633	668	IC Flow
53-1078	Alexa Fluor® 488 anti-human CD107b (LAMP-2)	488	519	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as LAMP-2 (lysosomal-associated membrane protein 2). CD107b, an approximately 105 kDa type I transmembrane protein, is highly glycosylated, and along with LAMP-1 (CD107a) is estimated to contribute to approximately 50% of all proteins of the lysosomal membrane. CD107b is expressed intracellularly on many cell types, and on the surface of activated platelets and T cells, as well as some cancer cells. Genetic deficiency of both CD107a and CD107b results in embryonic lethality. Loss of either CD107a or CD107b alone is not embryonic lethal, however the loss of CD107b causes a more severe phenotype. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-human CD107b (LAMP-2) has been reported for use in flow cytometric analysis.

Applications Tested

This eBioH4B4 (H4B4) antibody has been tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. This antibody has also been tested by immunoblotting at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000;406:906-910. (H4B4, FC, IHC, PubMed)

Kannan K, Stewart RM, Bounds W, Carlsson SR, Fukuda M, Betzing KW, Holcombe RF. Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium. Cell Immunol. 1996;171(1):10-19. (H4B4, FC, IHC, PubMed)

Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985 Jul;101(1):85-95.

Carlsson SR, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1988 Dec 15;263(35):18911-9.

Grutzkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8.

Eskelinen EL, Illert AL, Tanaka Y, Schwarzmann G, Blanz J, Von Figura K, Saftig P. Role of LAMP-2 in lysosome biogenesis and autophagy. Mol Biol Cell. 2002 Sep;13(9):3355-68.

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