Anti-Human CD107b (LAMP-2) Purified

Also known as: LAMP2, lysosomal-associated membrane protein 2

RUO: For Research Use Only

human CD107b antibody, Affinity Purified conjugate

Normal human peripheral blood cells (P) and Jurkat cell line (J) lysates were immunoblotted with 1 µg/ml of Anti-Human CD107b (LAMP-2) Purified and revealed with Anti-Mouse HRP.

Contents: Anti-Human CD107b (LAMP-2) Purified
Catalog Number: 14-1078
Concentration: 0.5 mg/ml
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 2-8°C.
Clone: eBioH4B4 (H4B4)
Host/Isotype: Mouse IgG1, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-1078-80	25 ug	To view relevant pricing, please select your country and resume browsing.
14-1078-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin FITC
12-4317	Streptavidin PE
14-1072	Anti-Mouse CD107b (LAMP-2) Purified (eBioABL-93 (ABL-93))
14-1079	Anti-Human CD107a (LAMP-1) Purified (eBioH4A3)
17-4317	Streptavidin APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-1078	Anti-Human CD107b (LAMP-2) FITC	488	518	IC Flow	RUO
13-1078	Anti-Human CD107b (LAMP-2) Biotin	N/A	N/A	IC Flow	RUO
14-1078	Anti-Human CD107b (LAMP-2) Purified	N/A	N/A	IC Flow WB	RUO
51-1078	Anti-Human CD107b (LAMP-2) Alexa Fluor® 647	633	668	IC Flow	RUO
53-1078	Anti-Human CD107b (LAMP-2) Alexa Fluor® 488	488	519	IC Flow	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-human CD107b (LAMP-2) has been reported for use in flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.)

Applications Tested

This eBioH4B4 (H4B4) antibody has been tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. This antibody has also been tested by immunoblotting at 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Betts MR, Koup RA. Detection of T-cell degranulation: CD107a and b. Methods Cell Biol. 2004;75:497-512.(H4B4, FC, degranulation assay, PubMed)

Grutzkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8. (H4B4, FC, PubMed)

Eskelinen EL, Illert AL, Tanaka Y, Schwarzmann G, Blanz J, Von Figura K, Saftig P. Role of LAMP-2 in lysosome biogenesis and autophagy. Mol Biol Cell. 2002 Sep;13(9):3355-68.

Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000;406:906-910. (H4B4, IHC, PubMed)

Carlsson SR, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1988 Dec 15;263(35):18911-9.

Copyright © 2000-2010 eBioscience, Inc.
Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.