Affinity Purified anti-human CD107b (LAMP-2)

RUO: For Research Use Only

human CD107b antibody, Affinity Purified conjugate

PBMCs (P) and Jurkat cells (J) were lysed and 10 µg total protein/lane was immunoblotted using anti-human LAMP2 (eBioH4B4) antibody at 1 μg/mL and revealed with anti-mouse HRP.

Contents: Affinity Purified anti-human CD107b (LAMP-2)
Catalog Number: 14-1078
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Clone: eBioH4B4 (H4B4)
Host/Isotype: Mouse IgG1, κ
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-1078-80	25 ug	To view relevant pricing, please select your country and resume browsing.
14-1078-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
12-4317	Streptavidin-PE (Phycoerythrin)
14-1072	Affinity Purified anti-mouse CD107b (LAMP-2) (eBioABL-93 (ABL-93))
14-1079	Affinity Purified anti-human CD107a (LAMP-1) (eBioH4A3)
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-1078	Fluorescein isothiocyanate (FITC) anti-human CD107b (LAMP-2)	488	518	IC Flow	RUO
13-1078	Biotin anti-human CD107b (LAMP-2)	N/A	N/A	IC Flow	RUO
14-1078	Affinity Purified anti-human CD107b (LAMP-2)	N/A	N/A	IC Flow WB	RUO
51-1078	Alexa Fluor® 647 anti-human CD107b (LAMP-2)	633	668	IC Flow	RUO
53-1078	Alexa Fluor® 488 anti-human CD107b (LAMP-2)	488	519	IC Flow	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-human CD107b (LAMP-2) has been reported for use in flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.)

Applications Tested

This eBioH4B4 (H4B4) antibody has been tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. This antibody has also been tested by immunoblotting at 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Betts MR, Koup RA. Detection of T-cell degranulation: CD107a and b. Methods Cell Biol. 2004;75:497-512.(H4B4, FC, degranulation assay, PubMed)

Grutzkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8. (H4B4, FC, PubMed)

Eskelinen EL, Illert AL, Tanaka Y, Schwarzmann G, Blanz J, Von Figura K, Saftig P. Role of LAMP-2 in lysosome biogenesis and autophagy. Mol Biol Cell. 2002 Sep;13(9):3355-68.

Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000;406:906-910. (H4B4, IHC, PubMed)

Carlsson SR, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1988 Dec 15;263(35):18911-9.