Product Information

Contents: Affinity Purified anti-mouse CD107b (LAMP-2)
Catalog Number: 14-1072
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioABL-93 (ABL-93)
Isotype: Rat IgG2a, κ

Intracellular staining of thioglycolate-induced peritoneal-exudate cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD107b (ABL-93) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1072	Affinity Purified anti-mouse CD107b (LAMP-2)	N/A	N/A	IC Flow IHC IP
13-1072	Biotin anti-mouse CD107b (LAMP-2)	N/A	N/A	IC Flow
11-1072	Fluorescein isothiocyanate (FITC) anti-mouse CD107b (LAMP-2)	488	518	IC Flow
51-1072	Alexa Fluor® 647 anti-mouse CD107b (LAMP-2)	633	668	IC Flow
53-1072	Alexa Fluor® 488 anti-mouse CD107b (LAMP-2)	488	519	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as LAMP-2 (lysosomal-associated membrane protein 2). CD107b, an approximately 120 kDa protein, is highly glycosylated and expressed intracellularly on myeloid cells and epithelial cells. It is reported that CD107b is identical to the mouse Mac-3 antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioABL-93 (ABL-93) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Fluorochrome conjugated eBioABL-93 (ABL-93) is recommended for use in intracellular flow cytometry.)

Applications Tested

This eBioABL-93 (ABL-93) antibody has been tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal-exudate cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985 Jul;101(1):85-95. (ABL-93, IP, IHC, PubMed)

Cha Y, Holland SM, August JT. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J Biol Chem. 1990 Mar 25;265(9):5008-13. (ABL-93, affinity-chromatography, PubMed)

Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112.

Related Products

Cat. 14-1078 Affinity Purified anti-human CD107b (LAMP-2) (clone eBioH4B4 (H4B4))
Cat. 14-4321 Affinity Purified Rat IgG2a Isotype Control