Contents: Affinity Purified anti-mouse CD107a (LAMP-1)
Catalog Number: 14-1071
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio1D4B (1D4B)
Isotype: Rat IgG2a, κ
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Staining of BALB/c thioglycolate-induced peritoneal exudate cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD107a (1D4B) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
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Description
The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosome-associated membrane protein-1 (LAMP-1). CD107a is a type I, integral lysosomal membrane protein expressed constitutively at high levels by all cells. The majority of CD107a is localised to the limiting membrane of late endosomes-lysosomes, and LAMP-1 and LAMP-2 contribute approximately 50% of all lysosomal membrane protein. CD107a consists of an extensively glycosylated lumenal domain, a transmembrane domain and a short cytoplasmic tail. LAMP-1 deficient mice are viable and fertile, however mice deficient in both LAMP-1 and LAMP-2 have an embryonic lethal phenotype. LAMP-1 has been implicated in a variety of cellular functions, including cancer metastasis, and is a marker for lysosomal storage disorders.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated eBio1D4B (1D4B) is recommended for use in intracellular flow cytometry.)
Applications Tested
This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Rohrer J, Schweizer A, Russell D, Kornfeld S.
The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 1996 Feb;132(4):565-76. (
1D4B, immunoelectron microscopy,
PubMed)
Gough NR, Zweifel ME, Martinez-Augustin O, Aguilar RC, Bonifacino JS, Fambrough DM.
Utilization of the indirect lysosome targeting pathway by lysosome-associated membrane proteins (LAMPs) is influenced largely by the C-terminal residue of their GYXXphi targeting signals.
J Cell Sci. 1999 Dec;112 ( Pt 23):4257-69. (
1D4B, IF,
PubMed)
Chen JW, Pan W, D'Souza MP, August JT.
Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells.
Arch Biochem Biophys. 1985 Jun;239(2):574-86. (
1D4B, CD107a purification,
PubMed)
Related Products
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Cat.
14-4321 Affinity Purified Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart