Product Information

Contents: Affinity Purified anti-rat CD106 (VCAM-1)
Catalog Number: 14-1060
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioMR106 (MR106)
Isotype: Mouse IgG1, κ

Staining of Lou rat bone marrow cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-rat CD106 (MR106) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and Streptavidin-PE (cat. 12-4317). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1060	Affinity Purified anti-rat CD106 (VCAM-1)	N/A	N/A	FC IH/F IP WB
16-1060	Functional Grade* Purified anti-rat CD106 (VCAM-1)	N/A	N/A	FC
13-1060	Biotin anti-rat CD106 (VCAM-1)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and Integrin α4β7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMR106 (MR106) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioMR106 (MR106) antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Williams AJ, Atkins RC, Fries JW, Gimbrone MA Jr, Cybulsky MI, Collins T. Nucleotide sequence of rat vascular cell adhesion molecule-1 cDNA. Biochim Biophys Acta. 1992 Jun 15;1131(2):214-6.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control