Product Information

Contents: Affinity Purified anti-mouse CD105 (Endoglin)
Catalog Number: 14-1051
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MJ7/18
Isotype: Rat IgG2a, κ

Staining of bEnd3 cells with 0.25 μg Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg Purified anti-mouse CD105 (MJ7/18) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1051	Affinity Purified anti-mouse CD105 (Endoglin)	N/A	N/A	FC IH/F IP WB
13-1051	Biotin anti-mouse CD105 (Endoglin)	N/A	N/A	FC
12-1051	Phycoerythrin (PE) anti-mouse CD105 (Endoglin)	488	575	FC
57-1051	Pacific Blue® anti-mouse CD105 (Endoglin)	402	421	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MJ7/18 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.

Applications Tested

The MJ7/18 antibody has been tested by flow cytometric analysis of mouse brain-derived endothelial (bEnd3) cells and splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ge, A. Z. and E. C. Butcher. 1994. Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene 138(1-2): 201-6.

Tokita D, Ohdan H, Onoe T, Hara H, Tanaka Y, Asahara T. Liver sinusoidal endothelial cells contribute to alloreactive T-cell tolerance induced by portal venous injection of donor splenocytes.Transpl Int. 2005 Feb;18(2):237-45.

Quintanilla M, Ramirez JR, et al. 2003. Expression of the TGF-beta coreceptor endoglin in epidermal keratinocytes and its dual role in multistage mouse skin carcinogenesis. Oncogene. 22(38):5976-85. (MJ7/18, IHC formalin-fixed and WB, PubMed)

Onoe T, Ohdan H, et al. 2005. Liver sinusoidal endothelial cells tolerize T cells across MHC barriers in mice. J Immunol. 175(1):139-46. (MJ7/18, IHC frozen, PubMed)

Korpanty G, Carbon JG, et al. 2007. Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature. Clin Cancer Res. 13(1):323-30. (MJ7/18, IHC frozen, PubMed)

Perez-Gomez E, Villa-Morales M, et al. 2007. A role for endoglin as a suppressor of malignancy during mouse skin carcinogenesis. Cancer Res. 67(21):10268-77. (MJ7/18, WB, PubMed)

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