Product Information

Contents: Affinity Purified anti-human CD104 (Integrin beta 4)
Catalog Number: 14-1049
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 439-9B
Isotype: Rat IgG2b

Staining of the A549 cell line with 0.5 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or 0.5 μg of Purified anti-human CD104 (439-9B) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Sav-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1049	Affinity Purified anti-human CD104 (Integrin beta 4)	N/A	N/A	FC IH/F IHC(Paraffin) IP WB
13-1049	Biotin anti-human CD104 (Integrin beta 4)	N/A	N/A	FC IH/F IHC(Paraffin)
51-1049	Alexa Fluor® 647 anti-human CD104 (Integrin beta 4)	633	668	FC IH/F IHC(Paraffin)
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 439-9B recognizes human CD104, also known as integrin β4. CD104 is a 202 kDa subunit that associates with integrin α6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 439-9B antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting under nonreducing conditions only, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

This 439-9B antibody has been tested by flow cytometric analysis of A549 cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Simon-Assmann P, Leberquier C, Molto N, Uezato T, Bouziges F, Kedinger M. Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin. J Cell Sci. 1994 Mar;107 ( Pt 3):577-87.(439-9B, IP, PubMed)

Falcioni R, Sacchi A, Resau J, Kennel SJ. Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue. Cancer Res. 1988 Feb 15;48(4):816-21. (439-9B, IP, WB, PubMed)

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Cat. 14-4031 Purified Rat IgG2b Isotype Control