Product Information

Contents: Affinity Purified anti-human CD103 (Integrin α_IEL chain)
Catalog Number: 14-1038
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: B-Ly7
Isotype: Mouse IgG1, κ
HLDA No.: IV B58 and V-A129

Staining of 3-day PHA activated normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD103 (B-Ly7) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1038	Affinity Purified anti-human CD103 (Integrin alpha-IEL chain)	N/A	N/A	FC IH/F
11-1038	FITC anti-human CD103 (Integrin alpha-IEL chain)	488	518	FC
12-1038	PE anti-human CD103 (Integrin alpha-IEL chain)	488	575	FC
51-1038	Alexa Fluor® 647 anti-human CD103 (Integrin alpha-IEL chain)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The B-Ly7 monoclonal antibody reacts with human CD103, the α_E integrin. CD103 non-covalently associates with integrin β₇. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported

For research use only, not for diagnostic or therapeutic use. B-Ly7 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The B-Ly7 antibody has been tested by flow cytometric analysis of 2-3 day PHA-activated human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

de Harven E, Soligo D, Christensen H. 1995. Immunogold labeling for the diagnosis of leukemia by transmission and scanning electron microscopy. Scanning Microsc. 9:1191-199
de Harven E, Christensen H, Poppema S, Scott JG. 1994. Immunogold labelling of leukemic hairy cells with the B-ly7 monoclonal antibody: an SEM and TEM study. Microsc Res Tech. 28:356-67
Visser L, Dabbagh L, Poppema S. 1992. Reactivity of monoclonal antibody B-ly7 with a subset of activated T cells and T-cell lymphomas. Hematol Pathol. 6:37-42
Thaler J, Dietze O, Faber V, Greil R, Gastl G, Denz H, Ho AD, Huber H. 1990. Monoclonal antibody B-ly7: a sensitive marker for detection of minimal residual disease in hairy cell leukemia. Leukemia. 4:170-6
Schwarting R, Dienemann D, Kruschwitz M, Fritsche G, Stein H. 1990. Specificities of monoclonal antibodies B-ly7 and HML-1 are identical. Blood. 75:320-1

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