Product Information

Contents: Affinity Purified anti-mouse CD103 (Integrin α_IEL chain)
Catalog Number: 14-1031
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 2E7
Isotype: Armenian Hamster IgG

Staining of mouse lymph node cells with APC anti-mouse CD8a (53-6.7) (cat. 17-0081) and 0.5 μg of Purified Armenian Hamster IgG Iso Cntrl (cat. 14-4888) (left) or 0.5 μg of Purified anti-mouse CD103 (2E7) (right) followed by Biotin Anti-Armenian Hamster IgG (cat. 13-4113) and Streptavidin-FITC (cat. 11-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1031	Affinity Purified anti-mouse CD103 (Integrin alpha-IEL chain)	N/A	N/A	FA FC IH/F IP
16-1031	Functional Grade* Purified anti-mouse CD103 (Integrin alpha-IEL chain)	N/A	N/A	FA FC
13-1031	Biotin anti-mouse CD103 (Integrin alpha-IEL chain)	N/A	N/A	FC
11-1031	FITC anti-mouse CD103 (Integrin alpha-IEL chain)	488	518	FC
12-1031	PE anti-mouse CD103 (Integrin alpha-IEL chain)	488	575	FC
17-1031	Allophycocyanin (APC) anti-mouse CD103 (Integrin alpha-IEL chain)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2E7 monoclonal antibody reacts with mouse CD103, the α_E integrin. CD103 non-covalently associates with integrin β₇ and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2E7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vitro functional studies. (Please use Functional Grade purified 2E7, cat. 16-1031, in functional assays.)

Applications Tested

The 2E7 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lefrancois, L., T. A. Barrett, et al. 1994. "Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells. Eur J Immunol 24(3): 635-40.

Suzuki R, Nakao A, et al. 2002. Localization of intestinal intraepithelial T lymphocytes involves regulation of alphaEbeta7 expression by transforming growth factor-beta. Int Immunol. 14(4):339-45. (IHC frozen, PubMed)

Related Products

Cat. 11-4111  FITC Anti-Armenian Hamster IgG
Cat. 13-4113  Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4888  Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)