Product Information

Contents: Affinity Purified anti-human ICAM-2 (CD102)
Catalog Number: 14-1029
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CBRIC2/2
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with 0.06 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.06 μg of Purified anti-human ICAM-2 (CBRIC2/2) (colored histogram) followed by PE Donkey Anti-Mouse IgG (cat. 12-4012). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1029	Affinity Purified anti-human ICAM-2 (CD102)	N/A	N/A	FA FC IHC IP WB
11-1029	Fluorescein isothiocyanate (FITC) anti-human ICAM-2 (CD102)	488	518	FC
12-1029	Phycoerythrin (PE) anti-human ICAM-2 (CD102)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CBRIC2/2 antibody has been reported for use in flow cytometric analysis, immunoblotting, immunoprecipitation, immunohistochemical staining, and functional assays.

Applications Tested

This CBRIC2/2 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The CBRIC2/2 antibody has also been found useful for immunoblotting of a human peripheral blood cell lysate, recognizing a protein of approximately 55 kD under non-reducing conditions. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

de Fougerolles AR, Stacker SA, Schwarting R, Springer TA. Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1. J Exp Med. 1991 Jul 1;174(1):253-67. (CBRIC2/2, FC, IP, FA, PubMed)

Diacovo TG, deFougerolles AR, Bainton DF, Springer TA. A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2. J Clin Invest. 1994 Sep;94(3):1243-51. (CBRIC2/2, FC, IP, FA, PubMed)

Butini L, De Fougerolles AR, Vaccarezza M, Graziosi C, Cohen DI, Montroni M, Springer TA, Pantaleo G, Fauci AS. Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation. Eur J Immunol. 1994 Sep;24(9):2191-5. (CBRIC2/2, FA, PubMed)

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