Product Information

Contents: Affinity Purified anti-human CD98hc (4F2)
Catalog Number: 14-0982
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 5E5
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD98hc (5E5) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0982	Affinity Purified anti-human CD98hc (4F2)	N/A	N/A	FC IP
11-0982	Fluorescein isothiocyanate (FITC) anti-human CD98hc (4F2)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 5E5 recognizes human CD98hc protein also called CD98 heavy chain, FRP-1 (fusion regulatory protein-1 ) or 4F2. CD98 is a disulfide-linked complex composed of a heavy chain (a Type II membrane protein) and a light chain of which there are more than six different chains (LAT1, LAT2, y+LAT1, y+LAT2, Asc-1, xCT). CD98 can associate directly or indirectly with β 1 integrins, EpCAM and CD147 (EMMPRIN). The function of CD98 is thought to involve amino acid transport, cell fusion concomitantly with increased plasma membrane expression and integrin mediated adhesion. Expression of CD98 is broad including non-hematopoietic cells. In peripheral blood cells, monocytes expression is high while lymphocytes have intermediate levels and granulocytes express very little.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 5E5 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This 5E5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Haynes BF, Hemler ME, Mann DL, Eisenbarth GS, Shelhamer J, Mostowski HS, Thomas CA, Strominger JL, Fauci AS. Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. J Immunol. 1981 Apr;126(4):1409-14.

Dalton P, Christian HC, Redman CW, Sargent IL, Boyd CA. Differential effect of cross-linking the CD98 heavy chain on fusion and amino acid transport in the human placental trophoblast (BeWo) cell line. Biochim Biophys Acta. 2007 Mar;1768(3):401-10.

Xu D, Hemler ME.Metabolic activation-related CD147-CD98 complex. Mol Cell Proteomics. 2005 Aug;4(8):1061-71

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