human CD95 (Fas, APO-1) antibody: purified antibody

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Contents: Affinity Purified anti-human CD95 (Fas/APO-1)
Catalog Number: 14-0959
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DX2
Isotype: Mouse IgG1, κ
HLDA No.: VI C64

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0959-80	25 ug	$85
14-0959-82	100 ug	$170

*International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD95 (DX2) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0959	FITC anti-human CD95 (Fas/APO-1)	488	518	FC
12-0959	PE anti-human CD95 (Fas/APO-1)	488	575	FC
13-0959	Biotin anti-human CD95 (Fas/APO-1)	N/A	N/A	FC
14-0959	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC IHC
15-0959	PE-Cy5 anti-human CD95 (Fas/APO-1)	488	670	FC
17-0959	Allophycocyanin (APC) anti-human CD95 (Fas/APO-1)	633	660	FC
57-0959	Pacific Blue® anti-human CD95 (Fas/APO-1)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. DX2 has also been reported in in vitro studies of Fas-induced apoptosis. EOS9.1 is recommended for induction of apoptosis in vitro.

Applications Tested

The DX2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

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