Product Information

Contents: Affinity Purified anti-human CD95 (Fas/APO-1)
Catalog Number: 14-0958
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: EOS9.1
Isotype: Mouse IgM, κ
HLDA No.: N/A

Jurkat cells were treated for 6 hours with medium alone (plot A) or 0.1 µg/ml Functional Grade Purified EOS9.1 (plot B). Induction of apoptosis in these cells was determined by staining with PI and anti-BrdU-FITC using the Apo-BrdU^TM kit (cat# 88-6671). As shown in plot B, approximately 60% of cells underwent Fas-induced apoptosis following antibody treatment.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0958	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC
16-0958	Functional Grade* Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The EOS9.1 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. EOS9.1 does not block binding of DX2, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The EOS9.1 antibody has been reported for use in flow cytometric analysis. EOS9.1 is also effective in inducing apoptosis in in vitro functional studies. (Please use Functional Grade purified EOS9.1, cat. 16-0958, in functional assays.)

Applications Tested

The EOS9.1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and has also been tested for its ability to induce apoptosis of Jurkat cells. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kishimoto, T., H. Kikutani, et.al., eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London

Related Products

Cat. 11-0959  FITC anti-human CD95 (Fas/APO-1) (clone DX2)
Cat. 12-0959  PE anti-human CD95 (Fas/APO-1) (clone DX2)
Cat. 13-0959  Biotin anti-human CD95 (Fas/APO-1) (clone DX2)
Cat. 14-0959  Affinity Purified anti-human CD95 (Fas/APO-1) (clone DX2)
Cat. 15-0959  PE-Cy5 anti-human CD95 (Fas/APO-1) (clone DX2)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4752  Affinity Purified Mouse IgM Isotype Control
Cat. 11-5790  Fluorescein (FITC) anti-mouse IgM (clone II/41)
Cat. 13-5790  Biotin anti-mouse IgM (clone II/41)