mouse CD95 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse CD95 (Fas/APO-1)
Catalog Number: 14-0951
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 15A7
Isotype: Mouse IgG1, κ

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14-0951-81

50 ug

14-0951-85

500 ug

Staining of BALB/c thymocytes with 0.5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.5 μg of Purified anti-mouse CD95 (15A7) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0951	Affinity Purified anti-mouse CD95 (Fas/APO-1)	N/A	N/A	FC
13-0951	Biotin anti-mouse CD95 (Fas/APO-1)	N/A	N/A	FC
12-0951	PE anti-mouse CD95 (Fas/APO-1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 15A7 antibody has been tested by flow cytometric analysis of BALB/c mouse splenocyte and thymocyte cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Yonehara, S. 1999. Effects of anti-Fas antibodies on lymphocytes and other organs: preparation of original and new monoclonal antibodies and amelioration of systemic autoimmune disease. Int Rev Immunol 18(4): 329-45.
Nishimura, Y., A. Ishii, et al. 1995. Expression and function of mouse Fas antigen on immature and mature T cells. J Immunol 154(9): 4395-403.

Related Products

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Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
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Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control