mouse CD94 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse CD94
Catalog Number: 14-0941
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 18d3
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-0941-81	50 ug
14-0941-82	100 ug
14-0941-85	500 ug

Surface staining of mouse splenocytes with anti-mouse CD94 (18d3) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0941	Affinity Purified anti-mouse CD94	N/A	N/A	FC
13-0941	Biotin anti-mouse CD94	N/A	N/A	FC
11-0941	FITC anti-mouse CD94	488	518	FC
12-0941	PE anti-mouse CD94	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 18d3 monoclonal antibody reacts with mouse CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells, a subset of T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage. DBA/2J mice are naturally CD94-deficient and do not express cell-surface CD94/NKG2A receptors, even on neonatal NK cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 18d3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 18d3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Vance, R. E., A. M. Jamieson, et al. (1999). "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." J Exp Med 190(12): 1801-12.
Vance, R. E., J. R. Kraft, et al. (1998). "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." J Exp Med 188(10): 1841-8.
Vance, R. E., D. M. Tanamachi, et al. (1997). "Cloning of a mouse homolog of CD94 extends the family of C-type lectins on murine natural killer cells." Eur J Immunol 27(12): 3236-41.

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