rat MHC Class II antibody: purified antibody

Contents: Affinity Purified anti-rat MHC Class II
Catalog Number: 14-0920
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HIS19
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0920-82	100 ug	$145

*International customers: Please contact your distributor for region specific pricing.

Surface staining of rat splenocytes with anti-rat MHC Class II (HIS19) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0920	FITC anti-rat MHC Class II	488	518	FC
12-0920	PE anti-rat MHC Class II	488	575	FC
13-0920	Biotin anti-rat MHC Class II	N/A	N/A	FC
14-0920	Affinity Purified anti-rat MHC Class II	N/A	N/A	FA FC IH/F IP
17-0920	Allophycocyanin (APC) anti-rat MHC Class II	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS19 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of mixed lymphocyte reaction. (Please use Functional Grade purified HIS19 in functional assays.)

Applications Tested

The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Stet, R.J.M., J. Rozing, et.al.. 1985. HIS19: a monoclonal antibody recognizing a class II polymorphic determinant only absent on RT1n class II antigens. Transplant. Proc. 17: 182901831.
Uyama, T., J. B. Winter, et al. 1993. Replacement of dendritic cells in the airways of rat lung allografts. Am Rev Respir Dis 148(3): 760-7.
Stet, R. J., C. Thomas, et al. 1985. Graft versus host disease in the rat: Ia expression on target tissues. Adv Exp Med Biol 186: 545-54.

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