human CD90 (Thy1, Thy-1) antibody: purified antibody

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Contents: Affinity Purified anti-human CD90 (Thy1, Thy-1)
Catalog Number: 14-0909
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio5E10 (5E10)
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0909-80	25 ug	$85
14-0909-82	100 ug	$185

*International customers: Please contact your distributor for region specific pricing.

Staining of human erythroleukemia (HEL) cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD90 (5E10) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0909	Fluorescein isothiocyanate (FITC) anti-human CD90 (Thy1, Thy-1)	488	518	FC
12-0909	Phycoerythrin (PE) anti-human CD90 (Thy1, Thy-1)	488	575	FC
13-0909	Biotin anti-human CD90 (Thy1, Thy-1)	N/A	N/A	FC
14-0909	Affinity Purified anti-human CD90 (Thy1, Thy-1)	N/A	N/A	FC IH/F IP WB
15-0909	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD90 (Thy1, Thy-1)	488	670	FC
17-0909	Allophycocyanin (APC) anti-human CD90 (Thy1, Thy-1)	633	660	FC
45-0909	PerCP-Cy5.5 anti-human CD90 (Thy1, Thy-1)	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested

This eBio5E10 (5E10) antibody has been tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med. 1993 May 1;177(5):1331-42. (5E10, mAb development, FC, WB, IP, PubMed)

Mayani H, Lansdorp PM. Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood. 1994 May 1;83(9):2410-7. (5E10, FC, PubMed)

Hung JT, Liao JH, Lin YC, Chang HY, Wu SF, Chang TH, Kung JT, Hsieh SL, McDevitt H, Sytwu HK. Immunopathogenic role of TH1 cells in autoimmune diabetes: evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model. J Autoimmun. 2005 Nov;25(3):181-92. (5E10, IHC, FC, PubMed)

Related Products

Cat. 14-0901    Affinity Purified anti-mouse CD90 (Thy-1, Thy1) (clone G7)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control