mouse CD90.2 (Thy-1.2, Thy1.2) antibody: purified antibody

Contents: Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)
Catalog Number: 14-0903
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 30-H12
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0903-81	50 ug	$50
14-0903-82	100 ug	$70
14-0903-85	500 ug	$140

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 0.06 μg of Purified Rat IgG2b Iso Cntrl (cat. 14-4331) (open histogram) or 0.06 μg of Purified anti-mouse CD90.2 (30-H12) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0903	FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	518	FC
12-0903	PE anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	575	FC
13-0903	Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC
14-0903	Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FA FC IH/F
16-0903	Functional Grade* Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 30-H12 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vivo and in vitro depletion. (Please use Functional Grade purified 30-H12, cat. 16-0903, in functional assays.)

Applications Tested

The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sugai M, Kondo S, Shimizu A, Honjo T. Isolation of differentially expressed genes upon immunoglobulin class switching by a subtractive hybridization method using uracil DNA glycosylase. Nucleic Acids Res. 1998 Feb 5;26(4):911-8. (In vitro depletion)

Ledbetter, J.A. and L.A. Herzenberg (1979). "Xenogenic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 11-0902    FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 12-0902    PE anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 13-0902    Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 14-0902    Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 15-0902    PE-Cy5 anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 17-0902    APC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4331    Affinity Purified Rat IgG2b Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)