Product Information

Contents: Affinity Purified anti-rat CD86 (B7-2)
Catalog Number: 14-0860
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 24F
Isotype: Mouse IgG1, κ

Surface staining of LPS stimulated rat splenocytes with anti-rat CD86 (24F) FITC (left), and PE (right). Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0860	FITC anti-rat CD86 (B7-2, B72)	488	518	FC
12-0860	PE anti-rat CD86 (B7-2, B72)	488	575	FC
14-0860	Affinity Purified anti-rat CD86 (B7-2, B72)	N/A	N/A	FA FC IHC IP
16-0860	Functional Grade* Purified anti-rat CD86 (B7-2, B72)	N/A	N/A	FC
13-0860	Biotin anti-rat CD86 (B7-2, B72)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 24F monoclonal antibody reacts with rat CD86, an ~90 kDa surface receptor, also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed by germinal center B cells and at low levels on macrophages, dendritic cells and non-lymphoid cells in the thymus. Its expression is upregulated on B cells through a variety of surface stimuli including LPS. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 24F antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported in blocking of CD86 in functional studies. (Please use Functional Grade purified 24F, cat. 16-0860, in functional assays.)

Applications Tested

The 24F antibody has been tested by flow cytometric analysis of resting and activated rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Maeda, K., T. Sato, et al. 1997. Characterization of rat CD80 and CD86 by molecular cloning and mAb. Int Immunol 9(7): 993-1000.
Damoiseaux, J. G., H. Yagita, et al. 1998. Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells. J Leukoc Biol 64(6): 803-9.
Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8

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