Product Information

Contents: Affinity Purified anti-human CD83
Catalog Number: 14-0839
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HB15e
Isotype: Mouse IgG1, κ
HLDA No.: IV T085

Staining of human dendritic cells with anti-human CD83 (HB15e) PE. Normal human monocytes were enriched and stimulated with recombinant human (rh) GM-CSF, rhIL-4 and rhTNFa for 7 days. Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0839	Affinity Purified anti-human CD83	N/A	N/A	FC
13-0839	Biotin anti-human CD83	N/A	N/A	FC
11-0839	FITC anti-human CD83	488	518	FC
12-0839	PE anti-human CD83	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HB15e antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HB15e antibody has been tested by flow cytometric analysis of human peripheral blood-derived dendritic cells. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zhou, L. J. and T. F. Tedder. 1996. CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells. Proc Natl Acad Sci U S A 93(6): 2588-92.
Zhou, L. J. and T. F. Tedder. 1995. A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells. Blood 86(9): 3295-301.
Zhou, L. J. and T. F. Tedder. 1995. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J Immunol 154(8): 3821-35.

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