Product Information

Contents: Affinity Purified anti-human CD82
Catalog Number: 14-0829
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 53H5
Isotype: Mouse IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0829	Fluorescein isothiocyanate (FITC) anti-human CD82	488	518	FC
14-0829	Purified anti-human CD82	N/A	N/A	FA FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 53H5 monoclonal antibody reacts with human CD82, a 50-53 kDa protein belonging to the tetra-span (TM4) transmembrane proteins. CD82 is expressed broadly by the majority of hematopoietic lineages, but not erythrocytes. Activation of T and B lymphocytes upregulates CD82 expression. CD82 is also expressed by epithelial and endothelial cells and fibroblasts.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 53H5 antibody has been reported for use in flow cytometric analysis. (Please use Functional Grade purified 53H5, cat. 16-0829, in functional assays.)

Applications Tested

This 53H5 antibody has been tested by flow cytometric analysis of human peripheral blood mononuclear cells. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shibagaki N, Hanada K, Yamaguchi S, Yamashita H, Shimada S, Hamada H. Functional analysis of CD82 in the early phase of T cell activation: roles in cell adhesion and signal transduction. Eur J Immunol. 1998 Apr;28(4):1125-33. (53H5, FA, PubMed)

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