Product Information

Contents: Affinity Purified anti-mouse CD81 (TAPA-1)
Catalog Number: 14-0811
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: Eat-2 (EAT2)
Isotype: Armenian Hamster IgG

Surface staining of mouse splenocytes with anti-mouse CD81 (Eat-2) Biotin followed by SA-PE. Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0811	Affinity Purified anti-mouse CD81 (TAPA-1)	N/A	N/A	FC IP WB
16-0811	Functional Grade* Purified anti-mouse CD81 (TAPA-1)	N/A	N/A	FC IP WB
13-0811	Biotin anti-mouse CD81 (TAPA-1)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The Eat-2 (EAT2) monoclonal antibody reacts with mouse CD81, a molecule also known as TAPA-1. CD81 is expressed on CD4⁺CD8⁺ thymocytes and broadly in the periphery with high level expressed by B cells, NK cells, macrophages and dendritic cells and lower level on resting T cells. Activation of T cells induces upregulation of CD81. CD81 is involved in homotypic adhesion of B and T cells as well as costimulation of T cells. The Eat-2 antibody is reported to cross-react with rat CD81.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Eat-2 (EAT2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

Applications Tested

The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Maecker, H. T., S. C. Todd, et al. (2000). "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." Hybridoma 19(1): 15-22.

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