Product Information

Contents: Affinity Purified anti-human CD74 (Invariant chain)
Catalog Number: 14-0749
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: LN2
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0749	Affinity Purified anti-human CD74 (Invariant chain)	N/A	N/A	FC IH/F IHC(Paraffin) IP
13-0749	Biotin anti-human CD74 (Invariant chain)	N/A	N/A	FC IH/F IHC(Paraffin)
11-0749	Fluorescein isothiocyanate (FITC) anti-human CD74 (Invariant chain)	488	518	FC IH/F IHC(Paraffin)
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The LN2 monoclonal antibody recognizes CD74 (MHC Class II-associated invariant chain), which is a 33-43 kDa nonpolymorphic type II membrane protein. CD74 is expressed on B cells, at lower levels on monocytes and macrophages, and on subsets of activated T cells, Langerhans cells, dendritic cells and epithelial cells. CD74 is known to act as a chaperone of MHC Class II (HLA-DR) proteins, promoting their exit from the ER and directing them to endocytic compartments. However, recently a signaling function has been demonstrated for CD74 based on its interaction with CD44 and its binding of the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 is also thought to play a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This LN2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

This LN2 antibody has been tested by flow cytometric analyis of normal human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028-36. (LN2, IHC (Paraffin), IP, PubMed)

Lamb CA, Yewdell JW, Bennink JR, Cresswell P. Invariant chain targets HLA class II molecules to acidic endosomes containing internalized influenza virus. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):5998-6002.

Becker-Herman S, Arie G, Medvedovsky H, Kerem A, Shachar I. CD74 is a member of the regulated intramembrane proteolysis-processed protein family. Mol Biol Cell. 2005 Nov;16(11):5061-9.

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