Product Information

Contents: Affinity Purified anti-mouse CD73 (Ecto-5'-nucleotidase)
Catalog Number: 14-0731
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioTY/11.8 (TY/11.8)
Isotype: Rat IgG1

Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.25 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (left) or 0.25 μg of Purified anti-mouse CD73 (TY/11.8) (right) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0731	Affinity Purified anti-mouse CD73 (Ecto-5'-nucleotidase)	N/A	N/A	FC IHC
16-0731	Functional Grade* Purified anti-mouse CD73 (Ecto-5'-nucleotidase)	N/A	N/A	FA FC
13-0731	Biotin anti-mouse CD73 (Ecto-5'-nucleotidase)	N/A	N/A	FC
12-0731	Phycoerythrin (PE) anti-mouse CD73 (Ecto-5'-nucleotidase)	488	575	FC
48-0731	eFluor^TM 450 anti-mouse CD73 (Ecto-5'-nucleotidase) (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

eBioTY/11.8 recognizes the with CD73 a 69-kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity. Expression on myeloid cells (CD11b) is restricted to the bone marrow. In human CD73 can be induced to secrete a soluble form with IL-2 suggesting a role in mediating activation signals. Differences between human and mouse CD73 have been reported. BALB/c mice have more CD4+CD73+ than CD8+CD73+ while the reciprocal is documented in humans.
CD73 is expressed on a subset of lymphocytes and increases during lymphocyte maturation. Recently it has been found that memory CD4 T cells express and are similar to the uncommitted primed precursor helper cells (Thpp) that can differentiate into Th1 or Th2 cells. Furthermore CD73 has been found on regulatory T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioTY/11.8 (TY/11.8) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

This eBioTY/11.8 (TY/11.8) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Eliopoulos N, Stagg J, Lejeune L, Pommey S, Galipeau J. Allogeneic marrow stromal cells are immune rejected by MHC class I- and class II-mismatched recipient mice. Blood. 2005 Dec 15;106(13):4057-65.

Kobie JJ, Shah PR, Yang L, Rebhahn JA, Fowell DJ, Mosmann TR. T Regulatory and Primed Uncommitted CD4 T Cells Express CD73, Which Suppresses Effector CD4 T Cells by Converting 5'-Adenosine Monophosphate to Adenosine. J Immunol. 2006 Nov 15;177(10):6780-6

Yamashita Y, Hooker SW, Jiang H, Laurent AB, Resta R, Khare K, Coe A, Kincade PW, Thompson LF. CD73 expression and fyn-dependent signaling on murine lymphocytes. Eur J Immunol. 1998 Oct;28(10):2981-90.

Yang L, Kobie JJ, Mosmann TR. CD73 and Ly-6A/E distinguish in vivo primed but uncommitted mouse CD4 T cells from type 1 or type 2 effector cells. J Immunol. 2005 Nov 15;175(10):6458-64.

Related Products

Cat. 14-4301  Affinity Purified Rat IgG1 Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)
Cat. 12-5773  PE anti-mouse/rat Foxp3 (clone FJK-16s)