Staining of 3-day PHA-stimulated human peripheral blood cells with 0.25 µg of Mouse IgG1 κ Isotype Control Purified (cat. 14-4714) (open histogram) or 0.25 µg of Anti-Human CD71 Purified (filled histogram) followed by Anti-Mouse IgG FITC (cat. 11-4011). Total viable cells were used for analysis.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This OKT9 (OKT-9) antibody has been tested by flow cytometric analysis of unstimulated and CD3/CD28-activated (3 days) human blood leukocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves M. 1981. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin. Proc Natl Acad Sci U S A. 78(7): 4515–4519.
Salcedo TW, Fleit HB. 1991. Plasma membrane and intracellular pools of transferrin receptors decline during in vitro cultivation of U937 cells. Cell Prolif. 24(4):383-401.
Wenning LA, Murphy RM. 1999. Coupled cellular trafficking and diffusional limitations in delivery of immunotoxins to multicell tumor spheroids. Biotechnol Bioeng. 62(5):562-75.
