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Contents: Affinity Purified anti-human CD71 (Transferrin Receptor) Catalog Number: 14-0717 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: T56/14 Isotype: Mouse IgG1

Surface staining of U937 cells with anti-human CD71 (T56/14) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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Description

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The T56/14 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The T56/14 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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The T56/14 antibody has been tested by flow cytometric analysis of 3-day PHA-activated human PBMC. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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