Product Information

Contents: Affinity Purified anti-human CD69
Catalog Number: 14-0699
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: FN50
Isotype: Mouse IgG1, κ
HLDA No.: IV A091

Surface staining of PHA stimulated human PBMC with anti-human CD69 (FN50) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0699	FITC anti-human CD69 (Very Early Activation Antigen, VEA)	488	518	FC
12-0699	PE anti-human CD69 (Very Early Activation Antigen, VEA)	488	575	FC
17-0699	Allophycocyanin (APC) anti-human CD69 (Very Early Activation Antigen, VEA)	633	660	FC
25-0699	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD69 (Very Early Activation Antigen, VEA)	488	760	FC
14-0699	Affinity Purified anti-human CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FC IHC
13-0699	Biotin anti-human CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FC

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Description

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The FN50 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

The FN50 antibody has been tested by flow cytometric analysis of resting and 6-hour TPA-activated human PBMC. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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