Product Information

Contents: Affinity Purified anti-mouse CD69 (Very Early Activation Antigen)
Catalog Number: 14-0691
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: H1.2F3
Isotype: Armenian Hamster IgG

Surface staining of ConA stimulated splenocytes with anti-mouse CD69 (H1.2F3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0691	Affinity Purified anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FA FC IHC IP
16-0691	Functional Grade* Purified anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FA FC
13-0691	Biotin anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FC
11-0691	FITC anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	518	FC
12-0691	PE anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	575	FC
15-0691	PE-Cy5 anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	670	FC
17-0691	Allophycocyanin (APC) anti-mouse CD69 (Very Early Activation Antigen, VEA)	633	660	FC
25-0691	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	760	FC
45-0691	PerCP-Cy5.5 anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The H1.2F3 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. H1.2F3 has also been reported in in vitro cell activation. (Please use Functional Grade purified H1.2F3, cat. 16-0691, in functional assays.)

Applications Tested

The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Yokoyama, W. M., F. Koning, et al. (1988). "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." J Immunol 141(2): 369-76.

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