Product Information

Contents: Affinity Purified anti-human CD68 (macrosialin)
Catalog Number: 14-0689
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioY1/82A (Y1/82A)
Isotype: Mouse IgG2b, κ

Intracellular staining of normal human peripheral blood cells with 1 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 1μg of Purified anti-human CD68 (Y1/82A) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0689	Affinity Purified anti-human CD68 (macrosialin)	N/A	N/A	FC IH/F
13-0689	Biotin anti-human CD68 (macrosialin)	N/A	N/A	FC
11-0689	Fluorescein isothiocyanate (FITC) anti-human CD68 (macrosialin)	488	518	FC
12-0689	Phycoerythrin (PE) anti-human CD68 (macrosialin)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.
Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioY1/82A (Y1/82A) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioY1/82A (Y1/82A) antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Davey F.R., J. Cordell, W.N. Erber, K.A.F Pulford, K.C. Gatter, D.Y. Mason. 1988. Monoclonal antibody (Y1/82A) with specificty towards peripheral blood monocytes and tissue macrophages. J. Clin. Pathol 41:753-758.

Strobl H, Scheinecker C, Csmarits B, Majdic O, Knapp W. Flow cytometric analysis of intracellular CD68 molecule expression in normal and malignant haemopoiesis. Br J Haematol. 1995 Aug;90(4):774-82.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control