Product Information

Contents: Affinity Purified anti-human CD61 (integrin β3)
Catalog Number: 14-0619
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: VI-PL2
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood platelets with 0.06 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (left) or 0.06 μg of Purified anti-human CD61 (VI-PL2) (right) followed by PE Donkey Anti-Mouse IgG (H+L) (cat. 12-4012). Samples were then extensively washed and co-stained with FITC anti-human CD42b (HIP1) (cat. 11-0429) to label platelets. CD42b(-) events represent cell debris.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0619	Affinity Purified anti-human CD61 (integrin beta 3)	N/A	N/A	FA FC IH/F WB
11-0619	Fluorescein isothiocyanate (FITC) anti-human CD61 (integrin beta 3)	488	518	FC
12-0619	Phycoerythrin (PE) anti-human CD61 (integrin beta 3)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin β3 and GPIIIa. CD61 is a 90-110 kDa member of the /beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells. CD61 binds non-covalently with the α integrins CD41 and CD51, to form the αIIbβ3 (CD41/CD61) and αvβ3 (CD51/CD61) complexes. Theseαβ heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin. The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This VI-PL2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining, and functional assays.

Applications Tested

This VI-PL2 antibody has been tested by flow cytometric analysis of human peripheral blood platelets. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Barrett L, Dai C, Gamberg J, Gallant M, Grant M. Circulating CD14-CD36+ peripheral blood mononuclear cells constitutively produce interleukin-10. J Leukoc Biol. 2007 Jul;82(1):152-60. (VI-PL2, FC, PubMed)

Roberts MS, Woods AJ, Dale TC, Van Der Sluijs P, Norman JC. Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins. Mol Cell Biol. 2004 Feb;24(4):1505-15. (VI-PL2, FA, PubMed)

Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK. VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76(3):1109-23. (VI-PL2, FA, PubMed)

Related Products

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Cat. 11-4011  FITC Anti-Mouse IgG
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Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
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