Product Information

Contents: Affinity Purified anti-mouse CD61
Catalog Number: 14-0611
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 2C9.G3
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 bone marrow cells with 0.25 µg FG Purified Armenian hamster IgG isotype control (cat.16-4444) (open histogram) or 0.25 µg Purified anti-mouse CD61 (2C9.G3) (colored histogram) followed by biotin anti-Armenian hamster IgG (cat.13-4113) and SAv-PE (cat.12-4317). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0611	Affinity Purified anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FA FC IH/F
16-0611	Functional Grade* Purified anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FA FC
13-0611	Biotin anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FC
11-0611	FITC anti-mouse CD61 (Integrin beta 3)	488	518	FC
12-0611	PE anti-mouse CD61 (Integrin beta 3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse CD61, also known as the integrin β₃. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin α subunits; α_V (CD51) to form Vitronectin Receptor and α_Iib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, cat. 16-0611, in functional assays.)

Applications Tested

The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Yasuda M, Hasunuma Y, Adachi H, Sekine C, Sakanishi T, Hashimoto H, Ra C, Yagita H, Okumura K. 1995. Expression and function of fibronectin binding integrins on rat mast cells. Int Immunol. 7:251-8.

Nohara K, Pan X, et al. 2005. Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes. J Immunol. 174(5):2770-7. (FC, PubMed)

Related Products

Cat. 11-4111    FITC Anti-Armenian Hamster IgG
Cat. 13-4113    Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4444    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 14-4888    Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)