Product Information

Contents: Affinity Purified anti-human CD56 (N-CAM, NCAM)
Catalog Number: 14-0568
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CB56
Isotype: Mouse IgG1

Normal human peripheral blood lymphocytes were stain with 1 µg Mouse IgG1 Isotype control (cat.14-4714) (left panel) or 1 µg anti-human CD56 antibody (CB56) (cat.12-0568)(right panel) followed by PE anti-mouse IgG antibody (cat.12-4012) and subsequently with APC anti-human CD56 (MEM188) (cat.17-0569).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0568	Affinity Purified anti-human CD56 (N-CAM, NCAM)	N/A	N/A	FC
13-0568	Biotin anti-human CD56 (N-CAM, NCAM)	N/A	N/A	FC
53-0568	Alexa Fluor® 488 anti-human CD56 (N-CAM, NCAM)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CB56 monoclonal antibody reacts with human CD56, also known as Neural cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and NKT cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CB56 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This CB56 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hayakawa Y, Huntington ND, Nutt SL, Smyth MJ. Functional subsets of mouse natural killer cells. Immunol Rev. 2006 Dec;214:47-55.

Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Related Products

Cat. 17-0569  Allophycocyanin (APC) anti-human CD56 (N-CAM, NCAM) (clone MEM188 (MEM-188))
Cat. 12-4012  Phycoerythrin (PE) Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (clone polyclonal)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control