Staining of normal human peripheral blood cells with anti-human CD55 (143-30) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Contents: Anti-Human CD55 Purified Catalog Number: 14-0559 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: 143-30 Host/Isotype: Mouse IgG1, κ HLDA Workshop: IV NL-7
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Size
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14-0559-80
25 ug
14-0559-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 143-30 monoclonal antibody reacts with human CD55, the 64-73 kDa molecule also known as DAF (Decay-Accelerating Factor). CD55 is a GPI-anchored single chain glycoprotein expressed on all cells in contact with serum including all hematopoietic cells and vascular endothelium; a soluble form of CD55 is also present in plasma and body fluids. CD55 is thought to play a role in the protection of cells from the damage by autologous complement via prevention of the amplification steps of the complement cascade. Antibodies against CD55 are mitogenic in the presence of phorbol esters.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 143-30 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 143-30 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
