Product Information

Contents: Affinity Purified anti-mouse CD54 (ICAM-1)
Catalog Number: 14-0542
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioKAT-1 (KAT-1, KAT1)
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD54 (KAT-1) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0542	Affinity Purified anti-mouse CD54 (ICAM-1)	N/A	N/A	FA FC IH/F IP
16-0542	Functional Grade* Purified anti-mouse CD54 (ICAM-1)	N/A	N/A	FA FC IH/F IP
12-0542	Phycoerythrin (PE) anti-mouse CD54 (ICAM-1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. (Please use Functional Grade purified eBioKAT-1 (KAT-1, KAT1), cat. 16-0542, in functional assays.)

Applications Tested

This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Seko Y, Matsuda H, Kato K, Hashimoto Y, Yagita H, Okumura K, Yazaki Y. Expression of intercellular adhesion molecule-1 in murine hearts with acute myocarditis caused by coxsackievirus B3. J Clin Invest. 1993 Apr;91(4):1327-36. (KAT-1, FA, PubMed)

Arai K, Sunamura M, Wada Y, Takahashi M, Kobari M, Kato K, Yagita H, Okumura K, Matsuno S. Preventing effect of anti-ICAM-1 and anti-LFA-1 monoclonal antibodies on murine islet allograft rejection. Int J Pancreatol. 1999 Aug;26(1):23-31. (KAT-1, PubMed)

Lehmann JC, Jablonski-Westrich D, Haubold U, Gutierrez-Ramos JC, Springer T, Hamann A. Overlapping and selective roles of endothelial intercellular adhesion molecule-1 (ICAM-1) and ICAM-2 in lymphocyte trafficking. J Immunol. 2003 Sep 1;171(5):2588-93. (KAT-1, FA, PubMed)

Related Products

Cat. 11-0541    FITC anti-mouse CD54 (ICAM-1) (clone YN1/1.7.4)
Cat. 12-0541    PE anti-mouse CD54 (ICAM-1) (clone YN1/1.7.4)
Cat. 13-0541    Biotin anti-mouse CD54 (ICAM-1) (clone YN1/1.7.4)
Cat. 14-0541    Affinity Purified anti-mouse CD54 (ICAM-1) (clone YN1/1.7.4)
Cat. 16-0541    Functional Grade Purified anti-mouse CD54 (ICAM-1) (clone YN1/1.7.4)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321    Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)