Also known as: Intercellular adhesion molecule-1, ICAM1
RUO: For Research Use Only
Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2a Istotype Control Purified (cat. 14-4321) (open histogram) or 0.25 µg of Anti-Mouse CD54 (ICAM-1) Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Mouse CD54 (ICAM-1) Purified Catalog Number: 14-0542 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: eBioKAT-1 (KAT-1, KAT1) Host/Isotype: Rat IgG2a, κ
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Cat. No.
Size
Price
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14-0542-81
50 ug
14-0542-82
100 ug
14-0542-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (Please use Functional Grade purified eBioKAT-1 (KAT-1, KAT1), cat. 16-0542, in functional assays.)
Applications Tested
This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Lehmann JC, Jablonski-Westrich D, Haubold U, Gutierrez-Ramos JC, Springer T, Hamann A.
Overlapping and selective roles of endothelial intercellular adhesion molecule-1 (ICAM-1) and ICAM-2 in lymphocyte trafficking. J Immunol. 2003 Sep 1;171(5):2588-93. (KAT-1, FA, PubMed)
Arai K, Sunamura M, Wada Y, Takahashi M, Kobari M, Kato K, Yagita H, Okumura K, Matsuno S.
Preventing effect of anti-ICAM-1 and anti-LFA-1 monoclonal antibodies on murine islet allograft rejection.
Int J Pancreatol. 1999 Aug;26(1):23-31.
Kasper M, Koslowski R, Luther T, Schuh D, Müller M, Wenzel KW. Immunohistochemical evidence for loss of ICAM-1 by alveolar epithelial cells in pulmonary fibrosis. Histochem Cell Biol. 1995 Nov;104(5):397-405. (KAT-1, IHC paraffin)
Seko Y, Matsuda H, Kato K, Hashimoto Y, Yagita H, Okumura K, Yazaki Y.
Expression of intercellular adhesion molecule-1 in murine hearts with acute myocarditis caused by coxsackievirus B3.
J Clin Invest. 1993 Apr;91(4):1327-36. (KAT-1, FA, PubMed)
