Product Information

Contents: Affinity Purified anti-human CD51/61
Catalog Number: 14-0519
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 23C6
Isotype: Mouse IgG1, κ
HLDA No.: IV P18

Staining of platelets with 0.5 µg of purified mouse IgG1 isotype control (cat.14-4714) (open histogram), or 0.5 µg of purified 23C6 followed by FITC anti-mouse IgG (cat. 11-4011) (colored histogram).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0519	Affinity Purified anti-human CD51/61	N/A	N/A	FC IH/F
16-0519	Functional Grade* Purified anti-human CD51/61	N/A	N/A	FA FC IH/F
13-0519	Biotin anti-human CD51/61	N/A	N/A	FC
11-0519	FITC anti-human CD51/61	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin αv/β3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other β subunits of the integrin family including β₁ (CD29), β₅ and β₆ to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 23C6 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 23C6 has also been reported in blocking of some adhesive processes. (Please use Functional Grade purified 23C6 in functional assays.)

Applications Tested

The 23C6 antibody has been tested by flow cytometric analysis of human melanoma cell line and peripheral blood. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control