Product Information
Contents: Affinity Purified anti-human CD49d (Integrin α4)
Catalog Number: 14-0499
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
Clone: 9F10
Isotype: Mouse IgG1, κ
HLDA No.: V S215
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Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human CD49d (9F10) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin α
4 subunit. The complex of CD49d non-covalently associated with integrin β
1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin β
7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 9F10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. 9F10 has also been reported in
in vitro functional assays. (Please use Functional Grade purified 9F10, cat.
16-0499, in functional assays.)
Applications Tested
The 9F10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
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