Product Information

Contents: Affinity Purified anti-human CD49d (Integrin α₄)
Catalog Number: 14-0499
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 9F10
Isotype: Mouse IgG1, κ
HLDA No.: V S215

Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human CD49d (9F10) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0499	PE anti-human CD49d (Integrin alpha 4)	488	575	FC
14-0499	Affinity Purified anti-human CD49d (Integrin alpha 4)	N/A	N/A	FA FC IHC
16-0499	Functional Grade* Purified anti-human CD49d (Integrin alpha 4)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin α₄ subunit. The complex of CD49d non-covalently associated with integrin β₁ (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin β₇ and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 9F10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. 9F10 has also been reported in in vitro functional assays. (Please use Functional Grade purified 9F10, cat. 16-0499, in functional assays.)

Applications Tested

The 9F10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control