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Product Information


Contents: Affinity Purified anti-human CD49d (Integrin α4)
Catalog Number: 14-0499
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 9F10
Isotype: Mouse IgG1, κ
HLDA No.: V S215
 
 
data image 1
Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human CD49d (9F10) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-0499 Affinity Purified anti-human CD49d (Integrin alpha 4) N/A N/A FA  FC  IHC 
16-0499 Functional Grade* Purified anti-human CD49d (Integrin alpha 4) N/A N/A FA  FC 
12-0499 PE anti-human CD49d (Integrin alpha 4) 488 575 FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description


The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin α4 subunit. The complex of CD49d non-covalently associated with integrin β1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin β7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).


Applications Reported


For research use only, not for diagnostic or therapeutic use. The 9F10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. 9F10 has also been reported in in vitro functional assays. (Please use Functional Grade purified 9F10, cat. 16-0499, in functional assays.)


Applications Tested


The 9F10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References



Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.


Related Products


Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control



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